Get Production of Membrane Proteins: Strategies for Expression PDF

By Anne Skaja Robinson

Designed as a research-level advisor to present recommendations and techniques of membrane protein construction at the small to intermediate scale, this practice-oriented booklet presents unique, step by step laboratory protocols in addition to a proof of the rules in the back of each one strategy, including a dialogue of its relative merits and downsides.
Following an introductory part on present demanding situations in membrane protein creation, the publication is going directly to examine expression platforms, rising equipment and methods, and protein particular considerations.
Case reports illustrate how one can pick out or pattern the optimum creation approach for any wanted membrane protein, saving either money and time at the laboratory in addition to the technical construction scale.
detailed in its assurance of "difficult" proteins with huge membrane-embedded domain names, proteins from extremophiles, peripheral membrane proteins, and protein fragments.

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Production of Membrane Proteins: Strategies for Expression and Isolation by Anne Skaja Robinson

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